New antibiotic research experiment--论文代写范文

51Due论文代写平台essay代写范文：“New antibiotic research experiment”，这篇论文主要描述的是由于人们对于抗生素的滥用，导致新型的病原局对于现有的抗生素产生了耐药性，原有的抗生素效果变得非常微小，需要去研究和寻找新的抗生素来替代现有的抗生素，对金银花的抗菌性和丁醇和氨苄青霉素进行研究分析。

Resistance to antibiotics by major bacterial pathogens has limited the efficacy of current antibacterial drugs, and thus it is necessary to search for new alternative compounds. In this study, the main objective was aimed to investigate the antimicrobial activity of Lonicera japonica methanolic crude extract and different sub-fraction (ethyl-acetate and butanol) extracts and its synergism activity with antibiotic (ampicillin).

The MIC values determined (Table 4.7) had demonstrated that the extracts of the Lonicera japonica flower possess antimicrobial activity. Methanol and butanol extracts exhibit moderate antimicrobial activity against S. epidermidis, E. coli, K. pneumonia and Serratia spp. with MIC values ranged between 12.5mg/mL and 25mg/mL; while ethyl acetate extract obtained relatively low MIC values, ranged from 6.25mg/mL to 12.5mg/mL against mentioned bacteria and thus displaying promising antimicrobial activity. In MBC test conducted, K. pneumonia was the most sensitive bacteria to ethyl-acetate extract with MBC value of 25mg/mL.

The TLC separation of the plant extracts showed good separation of the compounds and hence the antimicrobial activity of the separated compounds can be determined by TLC bioautography agar overlay method. The bioautography assay proved that plant extracts do contain active compounds that have antimicrobial activity as clear zone of inhibition was observed. In bioautography, clear zones of inhibition were recorded in ethyl-acetate extract on all of the tested bacteria; while for methanol extract, clear zone was only observed in the control spot on S. epidermidis, indicating the possibility that the bioactive compounds within methanol extract have synergistic activity among each other. It is recommended that further investigation into the compounds where clear zones of inhibition were recorded, to be conducted to determine the bioactive properties of that particular compound.

In synergism test, synergistic interactions of plant extracts with ampicillin were observed and results showed that, the combination of ampicilin with different plant extracts exhibited positive results. Such combination had significantly lowered both the MIC values of ampicilin and plant extracts against tested bacteria, including E. coli, K. pneumonia and Serratia spp. The strong synergism activity between the plant extracts and ampicillin proved the therapeutic potential of this plant. Since K. pneumonia was resistant to ampicilin (Table 4.3), results of this study may suggest that the Lonicera japonica extracts possess compounds with antimicrobial properties which can be used as antimicrobial agents in new drugs against drug-resistant bacteria.

It is thus concluded that Lonicera japonica do possess antimicrobial properties for at least certain type of potent microbes and this activity is largely dependent on the extracts used. The objectives of this study which were to determine the MIC and MBC value of Lonicera japonica plant extracts; test the antimicrobial properties of separated active compounds from plant extracts using TLC bioautography assay and investigate the synergism activity of the plant extracts with antibiotic were successfully achieved.

From the finding of antimicrobial activities of the plant under study, further work need to be done on the isolation, purification and structural elucidation of the bioactive compounds in these plants. Besides, there is a need to widen the extraction solvents choice of the plant, since the activity of the plant depend on the properties of the solvent used. Further study can be carried out to identify the compounds responsible for the enhancement of antibiotic activity. An elucidation of the mechanisms of action of these compounds must be followed by toxicity and in vivo tests to determine the therapeutic application of such compounds in combination. Based on the plant species traditional uses, additional pharmacological studies, such as anti-inflammatory activity could also be conducted.

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